Knowde Enhanced TDS
Identification & Functionality
Amiporine® ER Purified Pomegranate Extract Features & Benefits
Containing active anti-aging and dual action moisturizing agents, the purified pomegranate extract contained in Amiporine® ER offers several care benefits:
- Active anti-aging: Amiporine® ER is an anti-aging active ingredient to further densify, firm and rejuvenate the skin.
- Moisturizing active ingredient: The stimulation of aquaporin (essential proteins for water circulation in the skin) synthesis provides optimal hydration benefits.
- Firming active ingredient: The properties of the purified pomegranate extract Amiporine® ER increase the production of collagen by reducing collagenases, responsible for skin tissue relaxation.
Applications & Uses
Amiporine® ER can be used in the following products:
- Products for dry and dehydrated skin
- Products for mature skin
- Radiance skin care
- After-sun products
|Value||Units||Test Method / Conditions|
|Aerobic Bacteria||max. 100||CFU/g||-|
|Flash Point||max. 175||°C||-|
|Refractive Index||1.467 - 1.477||-||-|
Regulatory & Compliance
Technical Details & Test Data
Numerous tests have been carried out to validate the effectiveness of the purified pomegranate
Effect of Amiporine® increasing doses on aquaporins expression by keratinocytes
Keratinocytes used for these experiments are from HaCat cell line. One night after being cultured, the media is exchanged with a new one containing Amiporine®. After a 72-hour incubation, cultures are rinsed and fixed for immunostaining.
This experiment highlights the Amiporine® effect: aquaporins expression increases when the active concentration increases.
Aquaporins expression observed on skin cells cultures: effect of a cream containing Amiporine®
Aquaporins expression is widely stimulated in skin cells treated with the cream containing Amiporine®.
Consequences of Amiporine® activity on skin metabolism: communication between epidermis cells is optimal.
Re-establishing water flow in the epidermis allows the skin to restore its suppleness and beauty. This unequalled effect of Amiporine® is the result of a general improvement in cells metabolism. As an example, the communication between keratinocytes was studied.
The following test was performed using a specific fluorescent tracer: Lucifer Yellow, that penetrates in cells if they have first been artificially cut.
When entered in a cell, Lucifer Yellow can diffuse in neighbouring cells using gap junctions. These are involved in the skin tissue organisation and coherence since they favour cells communication.
The following pictures were taken using a fluorescent microscope. In this manner, the effect of Amiporine® on cell communication via gap junction was visualised.
By controlling water flow, Amiporine® favours cell communication: Amiporine® is an essential way to fight against ageing.
Amiporine® effect on collagen I and III and matrix collgenase synthesis by human dermal fibroblasts
In order to demonstrate the Amiporine® activity on human dermal fibroblasts via the epidermis, keratinocytes were previously incubated with different doses of the active. The medium obtained was then used for fibroblasts culture.
This experiment demonstrates that Amiporine® also acts on fibroblasts via the epidermis cells. The active stimulates collagen I and III synthesis and decreases collagenase synthesis. On the opposite of collagen that build the dermis matrix, collagenase damages it.
Human keratinocytes were cultured and incubated overnight with a standard culture medium. Supernatants were then eliminated and replaced by a new medium (control) or medium containing Amiporine®.
After a 6-hour incubation, supernatants were eliminated, cells were rinced and again incubated with a new medium during 24 hours. After this period, supernatants were centrifuged and filtered for fibroblasts culture.
Fibroblasts were cultured with keratinocytes supernatants.
After a 48 hours incubation, supernatants were filtered and freeze at -20°C. The measurement of collagenase using ELISA dosage was then performed.
The measurement of collagen I and III was performed by using a semi-quantitative method of fluorescent immuno-labelling : after the labelling of collagens I and III, cells were lysed and fluorescent supernatants were then quantified with a spectrofluorimeter (Fluoroscan).
Amiporine® increases the collagen production and decreases the collagenase production, leading to a limitative effect of skin ageing.
Hormonal variation and aquaporins
All their life long, women are subjected to several hormonal variations, particularly during menstrual cycle and on pregnancy
These changes widely alter cells behaviour and can lead to a dysfunction affecting the general aspect of the skin.
Our research team performed a test which demonstrate that an increase in a sporadic hormonal concentration such as the estradiol, can alter aquaporins expression. This experiment was realised thanks to a flow spectrofluorimeter: this high-performance device allows to label the cells with a specific fluorescent antibody and to analyse cells precisely, one by one.
The results obtained prove that Amiporine® is able to reverse the estradiol effects on aquaporins expression and even to stimulate it despite the hormone presence.
Entirely natural, without preservative and stabilised in glycerine, Amiporine® is the first moisturising and restructuring active recommended in all cosmetic products. Shaking up traditional ideas on skin moisturising, A.M.I. revolutionises the cosmetic skin care world by stimulating the skin metabolism with the more essential life element: water.
Amiporine® acts on the biological and chemical foundations, by favouring water flow in the skin. Perfectly moisturised and boosted, the skin reveals its brightness and shows a young, sooth and restful aspect.