Hispanagar D-1 LE AGAROSE

With an electroendosmosis value of ≤0.13, this agarose is suitable for Blotting tests. The presence of DNase and RNase is not detected.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Labeling Claims: Non-Toxic

Physical Form: Gel, Powder, Solid

Safety Data Sheet

Knowde Enhanced TDS

Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Labeling Claims
Features
  • Extraordinary mechanical resistance for more reliable and easier handling.
  • Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
  • Easy preparation of the gel by simple dilution in aqueous buffers either by standard boiling or microwaving.
  • Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
  • Excellent transparency of the gel and high visibility.
  • Exceptionally low absorption of staining agents.
  • Absence of toxicity (polyacrylamide is neurotoxic).

Applications & Uses

D-1 LE: with Low EEO. High electrophoresis mobility
  • Nucleic acid analytical and preparative electrophoresis.
  • Blotting.
  • Protein electrophoresis such as radial immunodiffusion.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%
Clarity (at 1.5%)max. 3NTU
DNA resolution (≥ 1000 bp)Finely resolved
End-Expiratory Occlusion0.05-0.13
Gel BackgroundVery low
Gel Strength (at 1.5%)min. 2500g/cm2
Gel Strength (at 1%)min. 1200g/cm2
Gelling Temperature (at 1.5%)36 ± 1.5°C
Melting Temperature (at 1.5%)88 ± 1.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.1%

Technical Details & Test Data

Test Data

Hispanagar D-1 LE AGAROSE - Test Data

D1-LE Agarose gels in IX TAE buffer A-0.75%, B-1%, C-1.25%
Marker: 1Kb ladder.
Electrophoresis conditions: submarine gel, 2 hours 30 min, 4.5 V/cm in 1X TAE buffer.

Ranges Of Separation For D-1 LE And D-1 LE GQT AGAROSE

Hispanagar D-1 LE AGAROSE - Ranges of Separation For D-1 Le And D-1 Le Gqt Agarose