Hispanagar D-2 LE AGAROSE

This agarose has a higher gel temperature than D1 agarose, which provides greater thermal stability to the gels. This product has low electroendosmosis (≤ 0.14). Its main applications are nucleic acid electrophoresis, protein electrophoresis, and preparation of agarose beads.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Labeling Claims: Non-Toxic

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Labeling Claims
Features
  • Extraordinary mechanical resistance for more reliable and easier handling.
  • Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
  • Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
  • Greater thermal stability due to high hysteresis (difference between gelling and melting temperatures).
  • Excellent transparency of the gels.
  • Excellent elasticity and flexibility of the gels.
  • Great capacity for derivatization and cross-linking, which allows coupling of enzymes, antigens and other substances to the gel structure.
  • Exceptionally low absorption of staining agents.
  • Absence of toxicity.

Applications & Uses

D-2 LE: with low EEO.
  • Nucleic acid electrophoresis.
  • Protein electrophoresis (immunoelectrophoresis and counterelectrophoresis).
  • Preparation of agarose beads.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%
Clarity (at 1.5%)max. 4NTU
DNA resolution (≥ 1000 bp)Finely resolved
End-Expiratory Occlusionmax. 0.14
Gel BackgroundVery low
Gel Strength (at 1.5%)min. 1200g/cm2
Gel Strength (at 1%)min. 900g/cm2
Gelling Temperature (at 1.5%)42 ± 1.5°C
Melting Temperature (at 1.5%)87 ± 1.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.2%