Hispanagar D-3 AGAROSE

D3 agarose has a very high molecular weight, much higher than other agarose, a characteristic that results from extremely high gel strength. The gel structure and exclusion limit are similar to D1 agarose.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Labeling Claims: Non-Toxic

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Labeling Claims
Features
  • Extraordinary mechanical resistance for more reliable and easier handling.
  • Possibility of varying pore size in accordance with particle size by modifying gel concentration.
  • Extremely high gel strength allowing for lower gel concentrations, enabling the product to be used not only with high molecular weight nucleic acids such as chromosomes, but also with large-sized particles such as viruses and ribosomes.
  • Greater thermal stability due to high hysteresis difference between gelling and melting temperatures).
  • Excellent transparency of the gels.
  • Exceptionally low absorption of staining agents.
  • Absence of toxicity.
  • Due to its high gel strength dissolution by autoclaving is recommended for best performance.

Applications & Uses

Applications
  • Conventional electrophoresis: can be used with a broad range of particle sizes by modifying gel concentration.
  • Pulsed Field Gel Electrophoresis: possible because of its high gel strength.
  • Precast Electrophoresis Gel: D-3 can be dissolved by autoclaving, removing risk of bacteriological contamination.
  • Agarose Beads: higher gel strength allows operation at higher pressure, permitting greater flow of liquids without damage to the beads.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%
Clarity (at 1.5%)max. 4NTU
DNA resolution (≥ 1000 bp)Finely resolved
End-Expiratory Occlusionmax. 0.13
Gel BackgroundVery low
Gel Strength (at 1.5%)min. 3000g/cm2
Gel Strength (at 1%)min. 1600g/cm2
Gelling Temperature (at 1.5%)36 ± 1.5°C
Melting Temperature (at 1.5%)88 ± 1.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.1%