Hispanagar D-5 AGAROSE

Linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic, added to a very low sulfate content, produces a strong intercatenary interaction, which generates a gel with a very high gel resistance and a high exclusion limit.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Labeling Claims: Non-Toxic

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Labeling Claims
Features
  • Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes, but also with large sized particles like viruses and ribosomes.
  • High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used.
  • Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
  • Greater thermal stability due to high hysteresis difference between gelling and melting temperatures).
  • Exceptionally low absorption of staining agents.
  • Absence of toxicity.

Applications & Uses

Applications
  • Conventional Electrophoresis: can be used in a wide range of concentrations.
  • Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.
  • Blotting.
  • Agarose Beads preparation.
  • Cell and enzyme immobilization.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.25%
Clarity (at 1.5%)max. 4NTU
DNA resolution (≥ 1000 bp)Finely resolved
End-Expiratory Occlusionmax. 0.12
Gel BackgroundVery low
Gel Strength (at 1.5%)min. 3200g/cm2
Gel Strength (at 1%)min. 1800g/cm2
Gelling Temperature (at 1.5%)36 ± 1.5°C
Melting Temperature (at 1.5%)88 ± 1.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.12%

Technical Details & Test Data

Test Data

As shown in the following photographs, D-5 Agarose is suitable for a wide variety of ranges, by modifying its concentration.

Hispanagar D-5 AGAROSE Test Data

Ranges Of Separation For D-5 AGAROSE

Hispanagar D-5 AGAROSE Ranges Of Separation For D-5 AGAROSE

Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges