Knowde Enhanced TDS
Identification & Functionality
- Chemical Name
- Pharma & Nutraceuticals Functions
- CAS No.
- 9012-36-6
- EC No.
- 232-731-8
- Technologies
Features & Benefits
- Benefit Claims
- Labeling Claims
- Features
- Extremely high gel strength allowing for lower gel concentrations (0.3%), enabling it to be used not only with high molecular weight nucleic acids, including chromosomes, but also with large sized particles like viruses and ribosomes.
- High electrophoretic mobility. DNA mobility is greater when compared with D-1LE. Electrophoresis times are reduced depending upon buffer and agarose concentration used.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis difference between gelling and melting temperatures).
- Exceptionally low absorption of staining agents.
- Absence of toxicity.
Applications & Uses
- Markets
- Applications
- Conventional Electrophoresis: can be used in a wide range of concentrations.
- Pulsed Field Gel Electrophoresis: because of its higher exclusion limit, larger molecules can be separated.
- Blotting.
- Agarose Beads preparation.
- Cell and enzyme immobilization.
Properties
- Typical Properties
Value | Units | Test Method / Conditions | |
Ash Content | max. 0.25 | % | — |
Clarity (at 1.5%) | max. 4 | NTU | — |
DNA resolution (≥ 1000 bp) | Finely resolved | — | — |
End-Expiratory Occlusion | max. 0.12 | — | — |
Gel Background | Very low | — | — |
Gel Strength (at 1.5%) | min. 3200 | g/cm2 | — |
Gel Strength (at 1%) | min. 1800 | g/cm2 | — |
Gelling Temperature (at 1.5%) | 36 ± 1.5 | °C | — |
Melting Temperature (at 1.5%) | 88 ± 1.5 | °C | — |
Moisture Content | max. 10 | % | — |
Sulfate Content | max. 0.12 | % | — |
Technical Details & Test Data
- Test Data
As shown in the following photographs, D-5 Agarose is suitable for a wide variety of ranges, by modifying its concentration.
- Ranges Of Separation For D-5 AGAROSE
-
Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower, therefore lower concentrations can be used to obtain similar separation ranges