Hispanagar LM AGAROSE

Low Melt Agarose (low melting point) recommended for the separation of fragments ≥1 000 bp in analytical electrophoresis. The low melting temperature (65°C) allows the agarose to melt without damaging the DNA double helix. The presence of DNase and RNase is not detected.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Physical Form: Gel, Powder, Solid

Safety Data Sheet
  • TypeDocument Name
View All Documents

Knowde Enhanced TDS

Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Benefit Claims
Features
  • Lower gel strength than standard agaroses. Even so, gels can be handled easily.
  • Higher clarity (gel transparency) than gels of standard agaroses.
  • Greater sieving capacity.
  • LM Agaroses are classified in three categories, depending on the degree of derivatization. Gelling/melting temperatures and gel strength are the most important differences

Applications & Uses

LM (LOW MELT): with the highest gelling/melting temperatures and gel strength.
  • Electrophoresis of DNA fragments ≥1000 bp.
  • Tissue and cell culture.
  • Viral plaque assays.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%
Clarity (at 1.5%)max. 4NTU
DNA resolution (≥ 1000 bp)Finely resolved
End-Expiratory Occlusionmax. 0.12
Gel BackgroundVery low
Gel Strength (at 1.5%)min. 500g/cm2
Gelling Temperature (at 1.5%)24-28°C
Melting Temperature (at 1.5%)max. 65.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.12%

Technical Details & Test Data

Ranges Of Separation For LM AND LM GQT AGAROSE

Hispanagar LM AGAROSE - Ranges of Separation For Lm And Lm Gqt Agarose

Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower; therefore lower concentrations can be used to obtain similar separation ranges.