Hispanagar LM SIEVE AGAROSE

Agarose with a very low melting temperature and a high resolution capacity for fragments ≤1 000 bp, especially for PCR products, between 200 and 800 bp with GQT (Genetic Quality Tested) quality control certificate. The presence of DNase and RNase is not detected.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Applications & Uses

Applications
  • Electrophoresis of DNA fragments ≤1000 bp.
  • In-Gel enzymatic processing (digestion, ligation, PCR).
  • Preparative electrophoresis.
  • Analysis and recovery of small DNA fragments for further applications.
Applications and Uses

This agarose is GQT (Genetic Quality Tested) certified. This ensures that In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction
steps.
LM SIEVE Agarose is ideal for digestion by agarase enzymes, making it very easy to recover small DNA fragments suitable for cloning or enzymatic processing.
LM SIEVE Agarose can be used at high concentrations, forming gels with excellent clarity and a higher sieving capacity than standard melting agaroses. Due to their
high gel strength, LM SIEVE Agarose gels are very easy to handle, even at concentrations as low as 2%.

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.3%
End-Expiratory Occlusionmax. 0.10
Gel Strength (at 4%)min.1000g/cm2
Gelling Temperature (at 4%)max. 35°C
Melting Temperature (at 4%)max. 65°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.12%

Technical Details & Test Data

Functional Tests
  • DNA resolution: bands appear sharp and finely resolved.
  • DNAse/RNAse activity: none detected.
  • DNA binding: none detected.
  • In-Gel enzymatic processing: passes test.
  • Enzymatic degradation by agarase: passes test.
  • Gel background: very low after EtBr staining.
Test Data

Hispanagar LM SIEVE AGAROSE Test Data

LM-SIEVE Agarose gels in 1X TBE buffer A-2%, B-3%, C-4%.
Markers: lane 1 - pBR322DNA.Mspl; lane 2 - øX174DNA. HaeIII.
Electrophoresis conditions: submarine gel, 2 hours 30 min., 4.5 V/cm in 1X TBE buffer