Hispanagar MS-12 AGAROSE

Agarose recommended for DNA analytical gels. At a 2% concentration, it is capable of separating 30-1 500 bp fragments. The presence of DNase and RNase is not detected and does not have DNA Binding properties.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Benefit Claims
Benefits

Gels made with MS-12 have higher gel strength than competitive products. The gel is exceptionally firm but still flexible when handled, minimizing the danger of cracking or breaking.
MS-12 has the same melting and gelling temperature as regular agaroses, allowing faster and easier preparation of gels. MS-12 also gives excellent resolution at concentrations of ≤1 %.
MS-12 Agarose is recommended for all analytical applications, especially when DNA is recovered for subsequent use in enzymatic procedures.

Applications & Uses

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.35%
Clarity (at 1.5%)max. 5NTU
End-Expiratory Occlusionmax. 0.12
Gel Strength (at 1.5%)min. 2000g/cm2
Gelling Temperature (at 4%)max. 40°C
Melting Temperature (at 4%)max. 93°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.11%

Technical Details & Test Data

Test Data

Hispanagar MS-12 AGAROSE Test Data

Functional Tests
  • DNA resolution: bands appear sharp and finely resolved.
  • DNAse/RNAse activity: none detected.
  • Gel background: very low after EtBr staining.
  • Blotting: very good transference for DNA fragments 154 – 2176 bp in 4 % gels.
  • DNA binding: very low.
Ranges Of Separation For MOLECULAR SCREEN AGAROSES

Hispanagar MS-12 AGAROSE - Ranges of Separation For Molecular Screen Agaroses

Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower; therefore lower concentrations can be used to obtain similar separation ranges.