Hispanagar MS-4 AGAROSE

Is the agarose with the highest molecular sieving capacity; able to separate fragments of very small size with differences of few base pairs. Its gelling / melting temperatures are found in the Low Melt agarose range. Its low viscosity allows to prepare gels of high concentration and therefore increase its sieving capacity.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Physical Form: Gel, Powder, Solid

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Identification & Functionality

Chemical Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

MS-4 type of agarose is unique to Hispanagar

At 3 % concentration, MS-4 Agarose gives a resolution of DNA fragments similar to gels made with polyacrylamide at concentrations of 8 %. While MS-4 may be dissolved carefully by microwaving, gels are best prepared by autoclaving.

Features
  • Excellent resolution of DNA fragments lower than 500 bp, especially smaller primer–sized fragments.
  • Forms a very clear, transparent gel, even at concentrations of 5% or higher.
  • Efficient mechanical handling at all concentrations.
  • The chances of gels breaking or cracking when handled are greatly minimized.

Applications & Uses

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.3%
Clarity (at 3%)max. 6NTU
End-Expiratory Occlusionmax. 0.12
Gel Strength (at 3%)min. 500g/cm2
Gelling Temperature (at 3%)max. 31°C
Melting Temperature (at 3%)max. 76°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.11%

Technical Details & Test Data

Test Data

Hispanagar MS-4 AGAROSE Test Data

Ranges Of Separation For MOLECULAR SCREEN AGAROSES

Hispanagar MS-4 AGAROSE - Ranges of Separation For Molecular Screen Agaroses

Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower; therefore lower concentrations can be used to obtain similar separation ranges.