Knowde Enhanced TDS
Identification & Functionality
- Chemical Name
- Pharma & Nutraceuticals Functions
- CAS No.
- 9012-36-6
- EC No.
- 232-731-8
- Technologies
Applications & Uses
- Markets
- Applications
- Analytical and preparative gel electrophoresis of small DNA fragments.
- In-Gel applications.
- Applications and Uses
Due to its low gelling/melting temperatures NuGel GQT Agarose is compatible with In-gel applications (enzymatic processing of nucleic acids directly in remelted agarose) thus, it is not necessary to recover DNA from agarose gels.
The low viscosity of NuGel GQT agarose makes it possible for gels of high concentrations, even 6%, to be prepared easily. At lower concentration (<2%) gels are fragile and difficult to handle, so special care must be taken when working. The best concentration range for
easy handling is 3-6%.
Properties
- Typical Properties
| Value | Units | Test Method / Conditions | |
| Ash Content | max. 0.45 | % | — |
| Clarity (at 4%) | max. 6 | NTU | — |
| End-Expiratory Occlusion | max. 0.13 | — | — |
| Gel Strength (at 4%) | min. 800 | g/cm2 | — |
| Gelling Temperature (at 4%) | max. 35 | °C | — |
| Melting Temperature (at 4%) | max. 65 | °C | — |
| Moisture Content | max. 10 | % | — |
| Sulfate Content | max. 0.12 | % | — |
Technical Details & Test Data
- Functional Tests
- Fine resolution: DNA fragments ≤ 1000 bp.
- DNase/RNase activity: none detected.
- DNA binding: none detected.
- In-Gel enzymatic processing: passes test.
- Enzymatic degradation by agarase: passes test.
- Gel background: very low after EtBr.
- Test Data
NuGEL GQT Agarose gels in 1X TBE buffer. A-2%, B-3%, C-4%.
Markers: lane 1-pBR322 DNA. MspI, lane-2-ØX174 DNA.HaeIII.
Electrophoresis conditions: submarine gel, 2 hours, 4.5 V/cm. in 1X TBE buffer.