Enhanced TDS
Knowde-enriched technical product data sheet
Identification & Functionality
- Chemical Name
- Ingredient Name
- Food Ingredients Functions
- Pharma & Nutraceuticals Functions
- CAS No.
- 9012-36-6
- EC No.
- 232-731-8
- Product Families
Features & Benefits
- Labeling Claims
- Features
- Extraordinary mechanical resistance for more reliable and easier handling.
- Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
- Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
- Greater thermal stability due to high hysteresis.
- Excellent transparency of the gels.
- Low absorption of staining agents.
- Absence of toxicity.
Applications & Uses
- Markets
- Applications
- Analytical and preparative gel electrophoresis for nucleic acids ≥ 1000 bp.
- Blotting assays.
- Recovery of DNA fragments for further applications (enzymatic processing or cloning).
Properties
- Typical Properties
Value | Units | Test Method / Conditions | |
Ash Content | max. 0.4 | % | - |
Clarity (at 1.5%) | max. 3 | NTU | - |
End-Expiratory Occlusion | 0.05-0.13 | - | - |
Gel Strength (at 1.5%) | min. 2500 | g/cm2 | - |
Gel Strength (at 1%) | min. 1200 | g/cm2 | - |
Gelling Temperature (at 1.5%) | 36 ± 1.5 | °C | - |
Melting Temperature (at 1.5%) | 88 ± 1.5 | °C | - |
Moisture Content | max. 10 | % | - |
Sulfate Content | max. 0.1 | % | - |
Regulatory & Compliance
- Certifications & Compliance
Technical Details & Test Data
- Test Data
-
D1-LE Agarose gels in IX TAE buffer A-0.75%, B-1%, C-1.25%
Marker: 1Kb ladder.
Electrophoresis conditions: submarine gel, 2 hours 30 min, 4.5 V/cm in 1X TAE buffer. - Functional Tests
- DNA resolution: bands appear sharp and finely resolved.
- DNAse/RNAse activity: none detected.
- DNA binding: none detected.
- Gel background: very low after EtBr staining.
- Inhibition to restriction enzymes and ligase: none detected.
- Ranges Of Separation For D-1 LE And D-1 LE GQT AGAROSE