OYC Americas Glucose-6-Phosphate Dehydrogenase, recombinant from Leuconostoc sp.(rG6PDH)

Identification & Functionality

Pharma & Nutraceuticals Functions

Enzyme

Technologies

Pharmaceuticals & Nutraceuticals

Product Families

Pharmaceuticals & Nutraceuticals — Excipients

Formulation & Processing Aids

Applications & Uses

Markets

Healthcare & Pharma

Applications

Healthcare & Pharma — Medical

Laboratory & Diagnostics

Preparation

Product Code：
Lyophilized powder (contains no ammonium sulfate)
IU per 1 mg powder is ＞400 units.

Properties

Physical Form

Powder,

Solid

Typical Properties

	Value	Units	Test Method / Conditions
Specific Activity	850.0	IU/mg protein	-

Contaminants

	Value	Units	Test Method / Conditions
Molecular Weight (gel filtration）	146.0	kD	-
Hexokinase	max. 0.0001	%	-
Phosphoglucose isomerase	max. 0.00005	％	-
Phosphoglucose dehydrogenase	max. 0.00001	％	-
Creatine kinase	max. 0.00001	%	-
Glutathione reductase	max. 0.00001	%	-
Phosphoglucomutase	max. 0.001	-	-
Myokinase	max. 0.001	%	-
Lactate dehydrogenase	max. 0.01	%	-
pH stability	pH 5.5 - 8.0（37, 1 week）	-	-
Thermal stability（pH 6.6, 10 min）	max. 45	℃	-
Optimum pH	7.5 - 8.0	-	-
Optimum temperature	45.0	℃	-
Km value	264 （NAD＋）, 45 （NADP+	μmol/L	-
Km value	206 （G-6-P, NAD+ -linked）	μmol/L	-
Km value	145 （G-6-P, NADP+ -linked）	μmol/L	-
Molecular Weight (SDS-PAGE）	55.0	kD	-

Technical Details & Test Data

Reaction Equation

D-Glucose-6-phosphate + NAD(P)+ = D-Glucono-1,5-lactone 6-phosphate + NAD(P)H

Assay Procedure

Spectrophotometric Method

1. Wavelength；340 nm
2. Light path length；1 cm
3. Temperature；30℃
4. Pipette the following reagents into a cuvette

• 2.80 mL Tris-HCl buffer（ 55 mmol/L, pH 7.8, 30℃）containing MgCl2（3.3 mmol/L）
• 0.10 mL NAD+ （60 mmol/L）
• 0.10 mL G-6-P（100 mmol/L）
• 0.02 mL rG-6-PDH（L.M.）（about 3 IU/mL）

Packaging & Availability

Packaging Type

Bulk

Packaging

OYC No.	Package
46857003	200 units
46854003	1,000 units
46854903	Bulk

 

Storage & Handling

Storage Condition

Below -20°C