Knowde Enhanced TDS
Identification & Functionality
- Pharma & Nutraceuticals Functions
- Technologies
Applications & Uses
- Markets
- Preparation
Product Code:GlDH-03
Lyophilized powder (contains no ammonium sulfate)
IU per 1 mg powder is approximately 30 units.
Properties
- Typical Properties
- Contaminants
Value | Units | Test Method / Conditions | |
Specific Activity | 10.0 | IU/mg protein | - |
Value | Units | Test Method / Conditions | |
Glucose-6-phosphate dehydrogenase | max. 0.1 | % | - |
Phosphogluconate dehydrogenase | max. 0.5 | % | - |
Glutamate dehydrogenase (NAD+) | max. 0.1 | % | - |
Glutathione reductase | max. 0.1 | % | - |
NADH oxidase | max. 0.01 | % | - |
Technical Details & Test Data
- Reaction Equation
L-Glutamate + H20 + NADP+ = 2-Oxoglutarate + NH3 + NADPH
- Assay Procedure
Spectrophotometric Method
- Wavelength :340 nm
- Light path length: 1 cm
- Temperature: 25℃
- Pipette the following reagents into a cuvette
- 2.85 mL Na-Pyrophosphate buffer(0.1 mol/L, pH 9.0) containing L-Glutamate(0.1 mol/L)
- 0.15 mL NADP+ (10 mmol/L)
- 0.02 mL GlDH(NADP)(about 3 IU/mL)
Packaging & Availability
- Packaging Type
- Packaging
OYC No. Package 46485003 150 units 46486003 600 units 46487003 3,000 units 46489903 Bulk
Storage & Handling
- Storage Condition
Below -20°C