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OYC Americas Glutamate Dehydrogenase (GLDH – Yeast)

Glutamate Dehydrogenase (GLDH – Yeast) is Sourced from Yeast in Lyophilized Powder form.

Functions: Enzyme

Technical Data Sheet
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Knowde Enhanced TDS

Identification & Functionality

Pharma & Nutraceuticals Functions

Applications & Uses

Preparation

Product Code:GlDH-03
Lyophilized powder (contains no ammonium sulfate)
IU per 1 mg powder is approximately 30 units.

Properties

Typical Properties
ValueUnitsTest Method / Conditions
Specific Activity10.0IU/mg protein-
Contaminants
ValueUnitsTest Method / Conditions
Glucose-6-phosphate dehydrogenasemax. 0.1%-
Phosphogluconate dehydrogenasemax. 0.5-
Glutamate dehydrogenase (NAD+)max. 0.1%-
Glutathione reductasemax. 0.1%-
NADH oxidasemax. 0.01%-

Technical Details & Test Data

Reaction Equation

L-Glutamate + H20 + NADP+ = 2-Oxoglutarate + NH3 + NADPH

Assay Procedure

Spectrophotometric Method

  1. Wavelength :340 nm
  2. Light path length: 1 cm
  3. Temperature: 25℃
  4. Pipette the following reagents into a cuvette
  • 2.85 mL Na-Pyrophosphate buffer(0.1 mol/L, pH 9.0) containing L-Glutamate(0.1 mol/L)
  • 0.15 mL NADP+ (10 mmol/L)
  • 0.02 mL GlDH(NADP)(about 3 IU/mL)

Packaging & Availability

Packaging Type
Packaging
OYC No. Package
46485003 150 units
46486003 600 units
46487003 3,000 units
46489903 Bulk

 

Storage & Handling

Storage Condition

Below -20°C