Knowde Enhanced TDS
Identification & Functionality
- INCI Name
- Ingredient Origin
- Cosmetic Ingredients Functions
- Technologies
Features & Benefits
- Benefit Claims
- Labeling Claims
Applications & Uses
- Markets
- Applications
- Application pH Range
- 2 - 9
- Use Level
- 1 - 3%
- Application Data
Photobiome™ C 01 can be formulated in many kinds of emulsions (O/W, W/O), as well as in aqueous gels, serums, and lotions, including hydro-alcoholic lotions with up to 50% of ethanol. We recommend adding it in the final step of the manufacturing process, preferably under 40°C, i.e., during the cooling phase.
After some time, a precipitate appears. Being the product a suspension of lysed cells in a liquid medium, this is completely normal and can be solved by shaking well before use.
No incompatibilities have been reported: it is compatible with a wide range of thickeners (e.g., from polyacrylates to natural thickeners such as xanthan gum); it is compatible with a wide variety of preservative systems, surfactants, sun filters (physical and chemical), pigments and with other ingredients commonly used in skin care and sun care formulations (Betaine, tocopherol, hyaluronic acid, etc). The addition of Photobiome™ C 01 does not affect the pH or the viscosity of final cosmetic formulations at the recommended use concentrations.
Photobiome™ C 01 may give a light yellow to light brown color when formulated at the highest range of recommended concentrations being this coloration less apparent in emulsions than in transparent gels and lotions.
A dosage of 1 - 3 % of Photobiome™ C 01 is recommended in final cosmetic formulations.
Properties
- Physical Form
- Appearance
- Yellowish to dark brown viscous suspension⁽¹⁾⁽²⁾
- Odor
- Characteristic
- Dispersible in
- Water, Ethanol Solutions (up to 50% of ethanol)
- Physico-Chemical Properties
- Microbiological Values
- Composition
- Note
⁽¹⁾This is a natural product, so color or transparency may evolve slightly after production without affecting its properties.
⁽²⁾After some time, a precipitate may appear. This can be solved by shaking well before use.
⁽³⁾According to ISO 17516:2014 (and according to USP Chapter 61 or EP Chapter 2.6.12) due to inherent variability of the plate count method results are considered out of limit if > 200 CFU/g. Our specific test report does not obviate the need to test products for the own intentions and purposes of your company.
- Solubility
The product Photobiome™ C 01 is dispersible in water and ethanol solutions up to 50% of ethanol.
- Recommended pH Range
Photobiome™ C 01 can be formulated at a pH range of 2.0 - 9.0 in final cosmetic formulations.
| Value | Units | Test Method / Conditions | |
| pH | 2.0 - 3.5 | - | IOCQ02/001 |
| Density | 1.10 - 1.30 | g/mL | IOCQ1012 |
| Polyphenolics | min. 50 | ppm | IOCQ02/51 |
| Cell Membrane Lipid Content | min. 10 | g/L | POCQ02/015 |
| Value | Units | Test Method / Conditions | |
| Total Aerobic Microbial Count | max. 100⁽³⁾ | cfu/g | UNE-EN ISO 21149 |
| Yeast and Mold Count | max. 10 | cfu/g | UNE-EN ISO 16212 |
| Escherichia coli | Absence | in 1g | UNE-EN ISO 21150 |
| Staphylococcus aureus | Absence | in 1g | UNE-EN ISO 22718 |
| Pseudomonas aeruginosa | Absence | in 1g | UNE-EN ISO 22717 |
| Candida albicans | Absence | in 1g | UNE-EN ISO 18416 |
| Value | Units | Test Method / Conditions | |
| Glycerin | 35.45 | % | - |
| Gossypium Herbaceum (Cotton) Seed Extract | 25.0 | % | - |
| Punica Granatum Seed Extract | 25.0 | % | - |
| Trehalose | 8.5 | % | - |
| Pentylene Glycol | 5.0 | % | - |
| Fructooligosaccharides | 0.5 | % | - |
| Water | 0.4 | % | - |
| Phytic Acid | 0.15 | % | - |
Regulatory & Compliance
- Certifications & Compliance
- Chemical Inventories
Technical Details & Test Data
- In vivo Testing
- Skin tolerance
This Patch test intended to confirm, in a panel of healthy human subjects, the skin compatibility of Photobiome™ C 01, after a single application under maximizing and
experimental conditions, creating a certain occlusion and favoriting the penetration of the product through the skin, according to the recommendations of Colipa (August 1997)
“guidelines for the assessment of human skin compatibility”. Under the experimental conditions adopted Photobiome™ C 01 is classified as NON-IRRITANT for skin.- Skin sensitization (Hypoallergenicity)
The HRIPT intended to confirm the skin compatibility and the absence of allergenic potential of several cosmetic products including Photobiome™ C 01, after repeated application to the skin under exaggerated experimental conditions creating a certain occlusion and favoriting the penetration of the product through the skin, according to an adaptation from the methodology described by Marzulli and Maibach (Human Repeated Insult Patch Test for delayed contact hypersensitivity). Under the experimental conditions adopted, Photobiome™ C 01 induced no reaction of irritation and has a very good skin compatibility. Moreover, no allergic reaction was detected, thus, Photobiome™ C 01 may be considered as hypoallergenic under this specific context.
- Photo-patch test
This Photo-Patch test intended to check the absence of phototoxic potential of Photobiome™ C 01, after a single application to the skin under exaggerated experimental
conditions. The product was applied, once, to the skin under the patch. After removal of the patch, the area was exposed to UVA radiation. Under the experimental conditions adopted, Photobiome™ C 01 did not exhibit any evidence of phototoxic potential.- In vitro Testing
- Evaluation of skin irritation
Evaluation of skin irritation potential (production of reversible damage to the skin following the application of a chemical) of Photobiome™ C 01 by measurement of cytotoxic effect on
reconstructed human epidermis (RHE) model according to the OECD method 439 (OECD guideline for the testing of chemicals – in vitro skin irritation). According to the experimental conditions of the study, the product Photobiome™ C 01 is considered NON-IRRITANT for the skin.- Evaluation of ocular irritation
Assessment of the ocular irritation potential of Photobiome™ C 01 by Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labeling for eye irritation or serious eye damage according to OECD 492. According to the experimental conditions of the study, Photobiome™ C 01 is considered as NOT requiring classification and labeling.
- Mutagenicity test
Assessment of mutagenic potential according to OECD 471 Bacterial Reverse Mutation Test(Ames test). In the performed experimental conditions, the product Photobiome™ C 01 is considered NON-MUTAGENIC nor PRO-MUTAGENIC.
Storage & Handling
- Shelf Life
- 18 months
- Storage Instructions
To be stored in hermetically closed vessels at a temperature of 2 - 8°C and protected from direct light and humidity.
- Shelf Life Information
18 months after production if kept at the recommended storage conditions and the original packaging. We recommend maintaining the container/package well closed as long as possible and observe good hygienic practices in handling.