Enhanced TDS
Knowde-enriched technical product data sheet
Identification & Functionality
- Country of Origin
- INCI Name
- Ingredient Name
- Ingredient Origin
- Food Ingredients Functions
- Pharma & Nutraceuticals Functions
- Ingredients
- Aleppo Pine Seed Extract
- Product Families
- Composition
Fatty oil with high content of polyunsaturated fatty acids, including linoleic acid (C18:2) and oleic acids ( > 80%) & vitamin E.
Features & Benefits
- Benefit Claims
- Benefit Claims (Health)
- Labeling Claims
- Food Ingredients Features
- Product Background
Oleopine AP-104 is a 100% natural active ingredient obtained from Aleppo Pine seeds using supercritical fluid extraction. Aleppo pine seeds are released from cones freshly matured usually between 8 - 10 weeks after their collection from its trees. Naturally distributed throughout the Mediterranean area, Aleppo Pine is considered the dominant pine species in the natural pine Mediterranean Forests. The seeds have been traditionally consumed since ancient times for food due to their health promoting benefits.
- Product Benefits
Rich in tocopherols and omega oils this ingredient has the following benefits:
- OLEOPINE AP-104 helps prevent premature skin aging for a more youthful, plump, and glowing appearance
- Antioxidant and HEV protection: Protects from HEV-induced damage in form of reactive oxygen species (ROS) decreases by 28%
- Oxidative stress protection
- Antioxidant and UVA protection
- Long term skin moisturizer: Increases skin hydration by 40%
- Anti-inflammatory effect
Applications & Uses
- Applications
- Food & Nutrition Applications
- Skin Care Applications
- Sun Care Applications
- Use Level
- 0.01 - 5%
- Field of Use
- In food supplements: The Aleppo pine seed extract contribute to a healthy heart diet. It is an important dietary component. It is suitable for use in food supplements due to its high linoleic acid content, among other things.
- In cosmetics: Our Oleopine extract revitalizes and nourishes the skin while protecting it from oxidative stress damage induced by blue light (HEV) and ultraviolet radiation (UVA). This effect has been proven in vitro in form of reactive oxygen species (ROS) decrease in human keratinocytes. Additional clinical studies have shown its long-term moisturizing property through a significant increase in skin hydration delicately and effectively. Used daily, Aleppo pine seed extract helps prevent premature skin aging for a more youthful, plump, and glowing appearance.
- Product Applications
- Anti-UV and blue light serums
- Sun protection
- Night creams
- Revitalizing day creams
- Moisturizing serums
- Skin balancing creams
- Nutritive anti-aging treatments
Properties
- Physical Form
- Solubility
- Taste
- Typical
- Appearance
- Clear yellow-white liquid
- Odor
- Characteristic
- Insoluble in
- Water, Alcohol
- Soluble in
- Fixed oil
- Specifications
- Aleppo pine CO2 - Total Extract Properties
- Heavy Metals Content: No heavy metals and inorganic compounds
- Total aerobic Germs: No reproducible microorganisms
| Value | Units | Test Method / Conditions | |
| Density (at 20°C) | 0.915 - 0.930 | - | - |
| Refractive Index (at 20°C) | 1.465 - 1.480 | - | - |
| Peroxide Value | max. 30 | meq O₂/kg | - |
| Acid Value | max. 10 | mg KOH/g | - |
| Flash Point | min. 100 | °C | - |
Regulatory & Compliance
- Certifications & Compliance
- Regulation
The product complies with the requirements of Regulation (EC) No.1334/2008 on flavorings and with the requirements of Regulation (EC) No.1223/2009 on cosmetic products in the currently valid version.
Technical Details & Test Data
- Aleppo pine CO2 Extraction
Aleppo Pine seeds that contain the precious lipids are extracted by CO2 supercritical to maximize these essential compounds.
- Vegetable Proteins
- Fibers
- Compost
- Extracts
- Flavors
- Colorants
- Fragrances
- In vitro Analysis - HEV Protection
Analysis of the antioxidant and protective effects of Oleopine, in counteracting oxidative stress induced by high-energy visible light (HEV), in human keratinocytes (HaCaT), through Reactive Oxygen Species (ROS) quantification.
In Vitro Test Methodology:
- HaCaT cells were cultured in the presence of Oleopine at 0.01% during 24 hours.
- After treatment, cells were exposed to HEV light to induce oxidative-stress and ROS accumulation during 30 minutes (aprox. dose 120 J/cm), and the effects of the treatment on this process were evaluated.
- ROS concentration was measured by spectrofluorimetry in the samples tested.
- To determined the non-toxic and effective concentrations, cell viability after treatment with Oleopine was first assessed in Human HaCaT keratinocytes through quantification by MTT assay.
ROS after HEV (HaCaT, 24h): When cells were previously treated with Oleopine for 24 hours, results indicated that treatment at 0.01% significantly decreased ROS levels by 28.4 ± 5.6%, compared to the irradiated control.
Fig. Bar graphs representing ROS accumulation in samples treated with Oleopine at 0.01 % during 24 hours, and HEV irradiation for 30 minutes, in HaCaT keratinocytes, normalized to the irradiated group.
**** Represents statistical significance with p-value < 0.0001.
The in vitro treatment with Oleopine during 24 hours in human keratinocytes (HaCaT), displays antioxidant and HEV-protective capabilities, through significant protection from HEV-induced damage, in form of reactive oxygen species (ROS) decrease.
- In vitro Analysis - UVA Protection
Analysis of the antioxidant and protective effects of Oleopine in counteracting oxidative stress induced by ultraviolet A (UVA) light, after 24 hours treatment in human keratinocytes (HaCaT), through Reactive Oxygen Species (ROS) quantification.
In Vitro Test Methodology:
- Previously, the effects on cell viability were assessed through MTT assay to determine non-toxic concentrations and proliferating capabilities.
- HaCaT cells were cultured in the presence of Oleopine at 0.01 %, selected from previous assay during 24 hours.
- After treatment, cells were exposed to UVA light to induce oxidative-stress and ROS accumulation during 30 minutes (aprox. dose 3 J/cm2), and the effects of the treatment on this process was evaluated.
- ROS concentration was measured by spectrofluorimetry.
ROS after UVA (HaCaT, 24h): When cells were previously treated with Oleopine for 24 hours, results indicated that treatment at 0.01% significantly decreased ROS levels by 22.0 ± 3.6 %, compared to the irradiated control.
Fig. Bar graphs representing ROS accumulation in samples treated with Oleopine at 0.01 % during 24 hours, and UVA irradiation for 30 minutes, in HaCaT keratinocytes, normalized to the irradiated group.
**** Represents statistical significance with p-value < 0.0001.
The in vitro treatment with Oleopine during 24 hours in human keratinocytes (HaCaT), displays antioxidant and protective capabilities, through significant protection from UVA-induced damage, in form of reactive oxygen species (ROS) decrease.
- Clinical Cosmetic Assessment - Skin Hydration
- 20 volunteers (Male / Female; Between 18 and 65 years) were submitted to 28-day topical treatment with Oleopine at 5% in the facial area (twice per day, morning and night).
- Before (Day 0) and after 28 days of treatment (Day 28), skin hydration was measured through Corneometer®
- Volunteer self-assessment data was collected at the end of the test & the data statistically analyzed.
Results: Treatment with Oleopine during 28 days significantly increased hydration by 40 %, comparing each volunteer with its own control at day 0 (paired Student’s t-test).
Fig. Graphical representation of the skin hydration levels quantified through Corneometer before (Day 0) and after (Day 28) topical application with OLEOPINE during 4 weeks in 20 human volunteers. Mean and Standard Error of the Median (SEM) are shown.
**** Represents statistical significance with p value < 0.0001.Volunteer Self evaluation Results: The results were subjectively confirmed through the self-assessment questionnaire, with positive results for most of the tested parameters, including cosmetic attributes, cosmetic effectiveness and consumer opinion.
For the following percentage of volunteers, it is considered that Oleopine has the following effects:
- 90% - Keeps the skin Hydrated throughout the day
- 95% - Moisturizes the skin in a delicate and effective way. Increases Elasticity and Firmness Brings Luminosity to the skin.
In a clinical trial:
- 90% of people were satisfied with the treatment and analyzed had hydrated skin throughout the day.
- 85% of people would use this extract and would recommend it.
After 4 weeks of use:
- 95% of people analyzed had significant improvement in elasticity and firmness of their skin after use.
- 75% of people agreed their skin was instantly firm and soft with a youthful look.
- 95% of people agreed their skin was instantly hydrated with a youthful, radiant glow.
- 95% of people experienced increased skin luminosity after 4 weeks of use.
- 95% of people agreed their skin was hydrated in a delicate way.
- Processing Conditions
- Warm-up 40°C (104°F) and mix before use.
- It can be added to the emulsion once the emulsion is formed at a temperature < 50°C.
Safety & Health
- Safety
- Single Patch Test: Non-irritant
- Good skin compatibility
Packaging & Availability
- Packaging Type
- Packaging
- Aluminum can 5 kg (Certified ONU)
- Aluminum can 32 kg (Certified ONU)
Storage & Handling
- Shelf Life
- 5 Years
- Storage Recommendations
Stable for at least 5 years when stored in its original package, tightly sealed in a cool (46 - 90ºF) and dry location out of heat and light. Storage under inert gas