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CBS Customized Brewing Solutions Exalase is produced by fermentation of a selected strain of Bacillus subtilis and contains alpha-acetolactate decarboxylase activity. In classical lager fermentations without the use of Exalase, alpha-acetolactate is slowly converted to diacetyl by rising the temperature at the end of main fermentation and diacetyl is reduced by yeast to acetoin during the maturation period. Exalase can be used to catalyze the decarboxylation of alpha-acetolactate directly to acetoin since the beginning of excretion of alpha-acetolactate by yeast. The maturation period could be eliminated as far as diacetyl is concerned.

Ingredient Name: Alpha-Acetolactate

Functions: Enzyme

End Uses: Beer

    Knowde Enhanced TDS

    Identification & Functionality

    Enzyme Type
    Ingredient Name
    Food Ingredients Functions
    Ingredients
    Alpha-Acetolactate
    Technologies

    Applications & Uses

    Food & Nutrition Applications
    Dosage
    • 1-2 ml per Hl of beer.
    Activity
    • 2000 ADU/ml. One ADU is the amount of enzyme which under standard conditions produces 1µmol of acetoin by decarboxylation of alpha-acetolactate.

    Properties

    Temperature
    • The optimal temperature for this enzyme is around 30-40°C and at the normal lager fermentation temperature the enzyme works at 25% of its maximal activity, but as the fermentation is a long process (several days) the enzyme can eliminate all the alpha-acetolactate.
    pH Effect
    • Optimum pH between 5.5 and 6. At pH 5 the enzyme works at 50% of its maximal activity, and at pH 4 at 25%.
    • The same remark as for optimal temperature can be made.

    Safety & Health

    Safety

    Exalase product complies with FAO/WHO JECFA and FCC recommended specifications for food grade enzymes, supplemented with maximum limits of 5 x 104/g for total viable count and 102/g for molds; the product is GRAS.

    Packaging & Availability

    Packaging Type
    Availability

    Exalase is avalaible in liquid form in polyethylene drums of 1-5-25 liters.

    Storage & Handling

    Storage

    Exalase will maintain the declared activity for at least 6 months when stored at 5°C.