Knowde Enhanced TDS
Identification & Functionality
- Enzyme Type
- Ingredient Name
- Food Ingredients Functions
- Ingredients
- Glucoamylase
- Technologies
Features & Benefits
- Food Ingredients Features
Applications & Uses
- Markets
- Applications
- Mode of Action
- The enzyme is an exo-1,4-alpha-glucosidase (1,4D-Glucan glucanohydrolase) that catalyzes the releasing of successive units of glucose from the end of the liquefied starch chains. The enzyme can hydrolyze the alpha-D-1,6 branches as well as the alpha-D-1,4 polymeric bonds of the starch. The final product is exclusively glucose.
- GLUCOZYME 2x is produced with a strain that does not generate transglucosidic activity. There is a strong connection between uric acidic and amyloglucodisic alpha-holoamylase that strengthens the grade of hydrolysis of the alpha-D-1,4 bonds. The enzymatic preparation has a very low level of protease activity.
- Application
- Syrup and Alcohol production from starch sources.
- A typical saccharification is carried out at 60ºC, pH 4.3 with holding time from 24 to 72 hours, the dosage is 0.25 – 0.75 Kg/MT of dry starch.
- Elimination of the starch in apple juice: 0.5 to 1.5 ml per Hl of juice.
- Glucose production from starch dextrins (syrups, vegetal milk ...) 0.25 to 0.5 kg per Tm of dry matter
- Brewing: In the mash to increase alcohol yield from dextrins at 1-3 Kg per MT of cereals
- Brewing: In fermentation tanks at 1-5 g/Hl for dry beer.
Properties
- Appearance
- Amber liquid
- Typical Properties
| Value | Units | Test Method / Conditions | |
| pH (20ºC) | 4.0 - 5.5 | - | - |
| Glucoamy 2X Activity | 400.0 | AGU/ml. | - |
Technical Details & Test Data
- Activity
One unit is defined as the quantity of enzyme that produces 1 mg of dextrose from hydrolyzed starch in one hour under the standard conditions of pH 4.3 and 60°C.
- Classification And Quality of The Enzyme
GLUCOZYME 2x is an Amyloglucosidase enzyme .Exo-1,4-alpha-D-glocosidic, number IUB 3.2.3.1 from the source of Aspergillus Niger. Because it is a consumable enzyme, it fulfills the requirements of the JECFA and the FCC.
- Profile Data
pH Effect
In tests carried out at 60° at different pHs, GLUCOZYME 2x has an optimal activity at pH 4.3 and a work margin of 3.5 - 6,0.
Temperature Effect
The tests carried out at pH 4.3 at different temperatures show an optimal activity at 65 – 70°C. However, for prolonged periods of time, 60°C is recommended.
Effect of the pH on the stability
A 0.1% solution of GLUCOZYME 2x was maintained at 60°C at various pH values for 60 minutes. Afterwards, the solution was cooled and its activity in standard conditions was observed.
Effect of the temperature on the stability
A 0.1% solution of GLUCOZYME 2x was maintained at various temperatures for 60 minutes. After cooling, its standard activity was tested using as a control an unheated enzyme.
The effect of the pH on the stability (30% maltodextrin, DE18; 60°C., 72 hours)
The effect of the temperature on the stability (30% maltodextrin, DE18; pH 4.3, 72 hours)
pH effect on activity (30% maltodextrin, DE18; 60°C.)
Temperature effect on activity (30% maltodextrin, DE18; pH 4.3)
Packaging & Availability
- Packaging Type
- Packaging
GLUCOZYME 2x comes in 25 kg opaque polyethylene bottles as well as 1000 L containers.
Storage & Handling
- Storage
- GLUCOZYME 2x can be stored at room temperature. In order to achieve the maximum stability, storage at 4°C is recommended.
- Avoid temperatures superior to 15°C.