Duchefa

Duchefa Cellulase R-10 is from Trichoderma Viride.1 unit (U) of Cellulase will release 1.0 µmole of glucose from carboxymethyl cellulose. Routinely used for the isolation of protoplasts, for its ability to degrade cell walls. Cellulase “Onozuka R-10” is often used in combination with Macerozyme R-10 (cat. no. M 8002).Beldman, G. et al., The cellulase of Trichoderma Viride, . J. Biochem., 146, 301-308, 1985. Potrykus, J., et al., Protoplasts: Isolation, culture, plant regeneration, 118, 549-578, 1986. Tewes, A., et al., High yield isolation and rapid recovery of protoplasts from suspension cultures of tomato (Lycopersicon esculentum), 113, 141-150, 1984.Evans, D.A. et al., Plant protoplast isolation and culture, Int. Rev. Cyt. Suppl, 16, 33-53, 1983.

Duchefa Macerozyme R-10 is well suited for the isolation of plant cells and is often used in combination with cellulase "Onozuka R-10" (Cat. no. C8001). A multi-component enzyme mixture containg the following enzyme activities.

Duchefa Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endo-polygalactu-ronase and endo-pectin lyase in high activity. S. Ishii and T. Yokotsuka, Purification and properties of endo-polygalacturonase from (aspergillus japonicus), (Agric.Biol. Chem, 36, 1885 (1972).

Duchefa Cellulase RS is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.