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"Duchefa Biochemie bv offers a whole range of plant tissue culture media, biochemicals, containers, bioreactors and alot of other products needed for micro-propagation. All media are produced in our own laboratories and custom made media are produced for many customers.

For ordering, please register yourselves as a customer to get a customer id-number. You can only order Duchefa Biochemie products via this customer number after our confirmation."

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Duchefa Cellulase R-10 is from Trichoderma Viride.1 unit (U) of Cellulase will release 1.0 µmole of glucose from carboxymethyl cellulose. Routinely used for the isolation of protoplasts, for its ability to degrade cell walls. Cellulase “Onozuka R-10” is often used in combination with Macerozyme R-10 (cat. no. M 8002).Beldman, G. et al., The cellulase of Trichoderma Viride, . J. Biochem., 146, 301-308, 1985. Potrykus, J., et al., Protoplasts: Isolation, culture, plant regeneration, 118, 549-578, 1986. Tewes, A., et al., High yield isolation and rapid recovery of protoplasts from suspension cultures of tomato (Lycopersicon esculentum), 113, 141-150, 1984.Evans, D.A. et al., Plant protoplast isolation and culture, Int. Rev. Cyt. Suppl, 16, 33-53, 1983.

Duchefa Cellulase R-10

Duchefa Macerozyme R-10 is well suited for the isolation of plant cells and is often used in combination with cellulase "Onozuka R-10" (Cat. no. C8001). A multi-component enzyme mixture containg the following enzyme activities.

Duchefa Macerozyme R-10

Duchefa Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endo-polygalactu-ronase and endo-pectin lyase in high activity. S. Ishii and T. Yokotsuka, Purification and properties of endo-polygalacturonase from (aspergillus japonicus), (Agric.Biol. Chem, 36, 1885 (1972).

Duchefa Pectolyase Y-23

Duchefa Cellulase RS is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Duchefa Cellulase RS

Duchefa

"Duchefa Biochemie bv offers a whole range of plant tissue culture media, biochemicals, containers, bioreactors and alot of other products needed for micro-propagation. All media are produced in our o

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