Knowde Enhanced TDS
Identification & Functionality
- Carrier
- INCI Name
- Ingredient Origin
- Cosmetic Ingredients Functions
- EINECS No.
- 231-791-2, 207-997-3
- Technologies
- Product Families
- Origin
- Propanediol - Vegetable
- Aqua - Mineral
- Lindera Strychnifolia Root Extract - Vegetable
Features & Benefits
- Benefit Claims
- Labeling Claims
- Product Background
HAIR ISSUE STUDIED BY GREENTECH
Hair loss, or alopecia, is a common disorder that affects people of all ages. It is characterized by the formation of very fine vellus hairs resulting from the miniaturization of the hair follicles, thereby leading to the appearance of baldness. The most common type (90%) is androgenetic alopecia. It concerns 28% of males between 18-29 years old and 53% of males after 40 years old. The pathophysiology of alopecia is multifactorial, including stress, nutrition, exposome, genetic predisposition, hormonal sensibility.
Microbiota also seems to play a key role in hair growth disorders. Finding balance both in bacteriobiota and mycobiota of the hair would then be a major step towards healthy hair.Currently, only two Food and Drug Administration (FDA)-approved hair growth-promoting drugs, namely Minoxidil and Finasteride, are available in the market. However, these drugs are of limited use, because of their side effects.
Aware of the adverse effects of current therapies and being at the forefront of innovation in terms of microbiota, Greentech designed a natural alternative strategy using a systemic approach.Actions
- Rebalances Bacteriobiota & Mycobiota
- Slows - Hair Loss
- Restored Hair Vitality & Density
- Improves WNT/ β-CATENIN Pathways
Molecules
- SESQUITERPENES : LINDERANE (0.5-2.0 %) & LINDERALACTONE (1.5-3.0 %)
- POLYPHENOLS (including Catechins & Tannins) : 20-35 %
Sourcing
Lindera Strychnifolia Roots Native from South East of Asia
« A Promoter of Longevity and an Elixir of Life »
Applications & Uses
- Hair Care Applications
- Cosmetic Use
- Scalp Microbiota Health
- Anti-Hair Loss Products
- Anti-Aging Hair Care
- Daily Hair Care
- Formulations
- Concentration for use: 1-2%
- pH for use: 4.0 - 6.0
- Caution for use: Add in formulations at 35-40°C, while cooling or at any time in cold preparation.
Properties
- Physical Form
- Odor
- Characteristic
- Appearance
- Liquid
- Soluble in
- Water, Propanediol
- Insoluble in
- 10% in water
- Typical Properties
- Physico-Chemical Properties
- Quantifications
- Microbiological Values
| Value | Units | Test Method / Conditions | |
| Natural Content | 15 | % | - |
| Natural Origin concentration | 100 | % | - |
| Value | Units | Test Method / Conditions | |
| Refraction Index | 1.420 - 1.460 | - | GT007 |
| Specific Gravity | 1.020 - 1.080 | - | GT008 |
| Value | Units | Test Method / Conditions | |
| Water Content | max. 10.0 | % | GT022 |
| Value | Units | Test Method / Conditions | |
| Detection of Enterobacteria | Absence | - | ISO 18415 |
| Enumeration of Mesophilic Aerobic Bacteria | max. 100 | ufc/mL - cfu/mL | ISO 21149 |
| Enumeration of Yeasts and Molds | max. 100 | ufc/mL - cfu/mL | ISO 16212 |
Regulatory & Compliance
- Certifications & Compliance
- REACH Status
INCI Concentration REACH Status Propanediol 85 – 93 % Propanediol registered
n°01-2119489383-28Aqua ≤ 10 % Water exempted annex IV Lindera Strychnifolia Root
Extract5 – 7 % Exempt: < 1 ton/year (dry matter)
Technical Details & Test Data
- Hair Follicle Growth Cycling And Hair Growth Pathways
The aim of our investigation is to normalize the Wnt signaling pathway, which plays a major role in the regeneration of hair follicle cells by acting on inhibitory proteins such as DKK-1, DKK-3 and sFRP-1.
- DKK : Dickkopf-Related Protein
- TGFß: Transforming Growth Factor Beta
- sFRP: Secreted Frizzled-Related Protein
Regeneration of the follicle is realized from a reservoir of stem cells that proliferate and differentiate under the effect of signaling pathways (Wnt/β-catenin). DKK-1 blocks the initiation of this phase, TGFβ improves Catagen phase and sFRP-1 blocks Wnt activation.
- Clinical Study - In Vivo
- HAIRILINE® IMPROVES HAIR NUMBER
- HAIRILINE® IMPROVES HAIR NUMBER AFTER 84 DAYS
One solution proposed against chronic alopecia is hair transplantation. Between 3000 and 5000 hairs are then transplanted.
With HAIRILINE® :
HAIRILINE® + 7% ~ + 7000 Hairs - SELF-ASSESSMENT
% of men reporting A SPEED UP OF THE GROWTH 81% A DECREASE OF HAIR IN BRUSH, ON CLOTHES AND PILLOWS 73% A STIMULATION OF HAIR GROWTH AND A SLOW DOWN OF FALL 77% A DENSIFICATION OF HAIR 81% 
Evaluation of hairiline® about hair properties
Hairiline® reduces hair loss and improves quality of hair
- CHARACTERIZATION OF ALOPECIA SCALP MICROBIOME
We first characterized the alopecia microbiota and secondly evaluate the effect of HAIRILINE®.
Simple blind test on 24 men (12 healthy & 12 type II & III Alopecia). Men age 49 years old. HAIRILINE® 1% versus placebo.
Twice daily application during 84 days. Measurements : 16S ribosomal RNA for bacteria & ITS1 (Internal Transcripted Spacer 1) DNA for fungiCHARACTERIZATION OF ANDROGENETIC ALOPECIA BACTERIOBIOTA (at Day 0)
At Phylum & Genus levels
- Comparable repartition between healthy and androgenic alopecia scalps (in agreement with Polak-Witka et al., 2019)
At Species level
- Lower mean reads of Staphylococcus epidermidis (p<0.05)
- Higher mean reads of : Cutibacterium acnes and Stenotrophomonas sp (p<0.05) in alopecia as compared to control, inducing microinflammation, keratinase secretion and therefore hair-loss.
CHARACTERIZATION OF ANDROGENETIC ALOPECIA MYCOBIOTA (at Day 0)
At Phylum level
- Lower abundance of Basidiomycota and higher abundance of Ascomycota as compared to control
At Species level
- Decrease of Malassezia globosa/Malassezia restricta ratio as compared to control.
At Genus level
- Lower proportion of Malassezia genus and increase of other bacterial genus inducing keratinase synthesis ans therefore acceleration of hair-loss.
EFFECT OF HAIRILINE® ON THE SCALP MICROBIOME
WITH HAIRILINE® (at D84) (BACTERIOBIOTA)
At Phylum & Genus levels
Hairiline® maintains bacterial diversity
RATIO C. ACNES / S. EPIDERMIDIS
At Species level
NUMBER OF STENOTROPHOMONAS
HAIRILINE® decreases the ratio of Cutibacterium acnes/Staphylococcus epidermidis
involved in androgenic pathogenesis . A decrease of Stenotrophomonas geniculata
was also observed.Hairiline® rebalances the bacteriobiota ratio of the scalp, linked to healthy scalp.
WITH HAIRILINE® (at D84) (MYCOBIOTA)
At Species level
RATIO M. GLOBOSA / M. RESTRICTA
HAIRILINE® rebalances the M. restrica/M. globosa ratio.
Hairiline® restores the scalp mycoecology.
- Proven Efficacy - In Vitro Test
HAIRILINE® MODULATES INHIBITORS OF THE MAIN SIGNALING PATHWAYS
FOR HAIR GROWTHDickkopf related protein-1 (DKK-1) is a protein and a potent antagonist of the Wnt signaling pathway. It induces keratinocytes apoptosis and catagen phase and is overexpressed in alopecia.
Dermal fibroblast cells from a healthy donor (54 years old). Treatment during 48h with HAIRILINE® at several concentrations. Protein quantification in the supernatant by ELISA. *** p<0.001 vs control.
DKK-1 (PG.μG OF PROTEINS-1)Hairiline® inhibits the secretion of the catagen inducer, dkk-1, limiting hair loss
Hair loss is the result of premature entry into catagen due to various causes: TGFβ-1 (Transforming Growth Factor 1) is a catagen inducer.
Dermal papilla cells from a healthy donor (76 years old).
Treatment during 24h with HAIRILINE® at several concentrations. Protein quantification in the supernatant by ELISA. ** p<0.01, *** p<0.001 vs control
TGFB-1 SECRETION (RELATIVE QUANTIFICATION)
- HAIRILINE® DECREASES VASOCONSTRICTION FACTOR RELEASE
Endothelin-1 (EDT-1) is a potent vasoconstrictor. It induces the regression of Anagen hair follicle.
Dermal microvascular endothelial cells from a healthy donor (30 years old).
Treatment during 24h with HAIRILINE® at several concentrations.
Protein quantification in the supernatant by ELISA. * p<0.05 vs control.VS CONTROL ENDOTHELIN-1 SECRETION (At 0.02%) -21%* Hairiline® reduces endothelin-1 secretion, improving blood supply to hair.
sFRP-1 protein is an inhibitor of Wnt signaling pathways, inducing hair-loss.
VS CONTROL sFRP-1 (At 0.2%) -21%* Hairiline® limits the secretion of tgfβ-1 & production of the wnt antagonist sfrp-1, reducing hair loss.
- HAIRILINE® IS BETTER THAN MINOXIDIL CONCERNING PAPILLA CELLS PROLIFERATION
Human follicle Dermal papilla cells (HFDPC).
Treatment during 48H with HAIRILINE® or MINOXIDIL at several concentrations.
Cell proliferation was evaluated by MTT method.
** p<0.01, **** p<0.001, vs control.Hairiline® has inhibitory effects on dkk-1 and tgfβ-1 secretion follicle dermal papilla cells: not found with minoxidil.
Safety & Health
- Toxicological Tests
Tests Method Tested Concentrat Results Skin Irritation Patch Test 1 % Not irritant Eye Irritation Het cam 1 % Pratically non irritant Sensitization HRIPT 1 % Very good skin compatibility Phototoxicity ISO
24442:20111 % Not phototoxic Mutagenicity test Ames test Product tested at 8
dilutionsNot mutagenic
Storage & Handling
- Shelf Life
- 24 months
- Storage
15 / +25°C
Keep in the original packaging, without opening, in the recommended storage conditions.
Advice before use : Agitation