Knowde Enhanced TDS
Identification & Functionality
- Pharma & Nutraceuticals Functions
- Technologies
Applications & Uses
- Markets
- Preparation and storage
- Product Code：ADH-01
Lyophilized powder (contains ammonium sulfate)………below －20℃
IU per 1 mg powder is approximately 100 units.- Product Code：ADH-05
50% Glycerol solution………－25℃～－15℃
IU per 1 ml solution is approximately 1,500 units
Properties
- Physical Form
- Typical Properties
- Contaminants
| Value | Units | Test Method / Conditions | |
| Specific Activity | 50.0 | IU/mg protein | - |
| Value | Units | Test Method / Conditions | |
| Alcohol dehydrogenase | max. 0.01 | max. | - |
| NADH oxidase | max. 0.01 | % | - |
| β-Hydroxysteroid dehydrogenase | max. 2 | - | - |
Technical Details & Test Data
- Reaction Equation
Androsteron + NAD(P)+ ＝ 5α-Androsteron-3, 17-dione + NAD(P)H
- Assay Procedure
Spectrophotometric Method
- Wavelength；340 nm
- Light path length；1 cm
- Temperature；25℃
- Pipette the following reagents into a cuvette
2.75 mL Na-pyrophosphate buffer（10.9 mmol/L, pH 8.8), containing Ethanol（1.13 mol/l）
0.25 mL NAD+（10 mmol/l）
0.02 mL ADH（about 3 IU/ml）- Assay Procedure
Spectrophotometric Method
- Wavelength: 340 nm
- Light path length: 1 cm
- Temperature: 25℃
- Pipette the following reagents into a cuvette
- 0.60 mL Sodium pyrophosphate buffer （0.1 mol/L, pH 8.9）
- 2.10 mL Distilled water
- 0.20 mL NAD+ （6 mmol/L）
- 0.10 mL Androsterone（0.015% in methanol）
- 0.02 mL r3α-HSDH（about 1 IU/mL）
Packaging & Availability
- Packaging Type
- Packaging
Catalog Number Pack Size 46565005 25 units 46566005 100 units 46567005 500 units 46565905 10 ku, 25 ku, 250 ku, 1000 ku - Packaging
Lyophilized
OYC No. Package 46410001 15,000 units 46411001 75,000 units 46412001 300,000 units 46409901 Bulk
Glycerol solutionOYC No. Package 46410005 15,000 units 46411005 75,000 units 46412005 300,000 units 46409905 Bulk
Storage & Handling
- Storage Condition
Below -25°C~ -15°C