Knowde Enhanced TDS
Identification & Functionality
- Pharma & Nutraceuticals Functions
- Technologies
Applications & Uses
- Markets
- Preparation
Product Code：Diaphorase-03 Lyophilized powder (contains no ammonium sulfate)
IU per 1 mg powder is approximately 300 units.
Properties
- Typical Properties
| Value | Units | Test Method / Conditions | |
| Specific Activity (at 25°C and pH 7.5) | 20.0 | IU/mg protein | - |
Technical Details & Test Data
- Reaction Equation
NAD(P)H + acceptor ＝ NAD(P)+ ＋ reduced acceptor
- Reference Data
- Assay Procedure
Spectrophotometric Method
- Wavelength；600 nm
- Light path length；1 cm
- Temperature；25℃
- Pipette the following reagents into a cuvette
- 2.75 mL Potassium phosphate buffer （0.1 mol/L, pH 7.5） 0.10 mL NADH（9 mmol/L）dissoluted in Tris （10 mmol/L）
- 0.10 mL 2, 6-dichlorophenolindophenol（2.7 mmol/L）
- 0.05 mL Diaphorase（about 2.5 U/mL）
Packaging & Availability
- Packaging Type
- Packaging
OYC No. Package 46445003 1,000 units 46446003 10,000 units 46445903 Bulk
Storage & Handling
- Storage Condition
Below -20°C