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OYC Americas Glucose-6-Phosphate Dehydrogenase (G-6-P DH – LM))

Glucose-6-Phosphate Dehydrogenase (G-6-P DH – LM)) is Sourced from Leuconostoc Mesenteroides lyophilized powder form.

Functions: Enzyme

Technical Data Sheet
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Knowde Enhanced TDS

Identification & Functionality

Pharma & Nutraceuticals Functions

Applications & Uses

Application

Suitable enzymatic determination of glucose or ATP, CK-NAC, CK-MB, and TDM Assays.

Preparation

Product Code:G-6-PDH-93 Lyophilized powder (contains no ammonium sulfate)

IU per 1 mg powder is approximately 600 units

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Specific Activity400.0IU/mg protein-
Contaminants
ValueUnitsTest Method / Conditions
Hexokinasemax. 0.0001%-
Phosphoglucose isomerasemax. 0.00005-
Creatine kinasemax. 0.00001%-
Glutathione reductasemax. 0.00001%-
Phosphoglucomutasemax. 0.001--
Myokinasemax. 0.001%-
Lactate dehydrogenasemax. 0.01%-
Phosphogluconate dehydrogenasemax. 0.00001-

Technical Details & Test Data

Reaction Equation

D-Glucose 6-Phosphate + NAD(P)+ = D-Glucono-1, 5-lactone 6-phosphate + NAD(P)H

Assay Procedure

Spectrophotometric Method

  1. Wavelength: 340 nm
  2. Light path length: 1 cm
  3. Temperature: 30℃
  4. Pipette the following reagents into a cuvette
  • 2.80 mL Tris-HCl buffer(55 mmol/L, pH 7.8)containing MgCl2(3.3 mmol/L)
  • 0.10 mL NAD+(60 mmol/L)
  • 0.10 mL G-6-P(0.1 mol/L)
  • 0.02 mL G-6-PDH(L.M.)(about 3 IU/mL)

Packaging & Availability

Packaging Type
Packaging
OYC No. Package
46535083 200 units
46536083 1,000 units
46536903 Bulk

 

Storage & Handling

Storage Condition

Below -20°C