Knowde Enhanced TDS
Identification & Functionality
- Pharma & Nutraceuticals Functions
- Technologies
Applications & Uses
- Markets
- Application
Suitable enzymatic determination of glucose or ATP, CK-NAC, CK-MB, and TDM Assays.
- Preparation
Product Code:G-6-PDH-93 Lyophilized powder (contains no ammonium sulfate)
IU per 1 mg powder is approximately 600 units
Properties
- Typical Properties
- Contaminants
Value | Units | Test Method / Conditions | |
Specific Activity | 400.0 | IU/mg protein | - |
Value | Units | Test Method / Conditions | |
Hexokinase | max. 0.0001 | % | - |
Phosphoglucose isomerase | max. 0.00005 | % | - |
Creatine kinase | max. 0.00001 | % | - |
Glutathione reductase | max. 0.00001 | % | - |
Phosphoglucomutase | max. 0.001 | - | - |
Myokinase | max. 0.001 | % | - |
Lactate dehydrogenase | max. 0.01 | % | - |
Phosphogluconate dehydrogenase | max. 0.00001 | % | - |
Technical Details & Test Data
- Reaction Equation
D-Glucose 6-Phosphate + NAD(P)+ = D-Glucono-1, 5-lactone 6-phosphate + NAD(P)H
- Assay Procedure
Spectrophotometric Method
- Wavelength: 340 nm
- Light path length: 1 cm
- Temperature: 30℃
- Pipette the following reagents into a cuvette
- 2.80 mL Tris-HCl buffer(55 mmol/L, pH 7.8)containing MgCl2(3.3 mmol/L)
- 0.10 mL NAD+(60 mmol/L)
- 0.10 mL G-6-P(0.1 mol/L)
- 0.02 mL G-6-PDH(L.M.)(about 3 IU/mL)
Packaging & Availability
- Packaging Type
- Packaging
OYC No. Package 46535083 200 units 46536083 1,000 units 46536903 Bulk
Storage & Handling
- Storage Condition
Below -20°C