OYC Americas Glutamate Dehydrogenase (NADP), recombinant from bacteria (rGlDH)

Identification & Functionality

Pharma & Nutraceuticals Functions

Enzyme

Technologies

Pharmaceuticals & Nutraceuticals

Product Families

Pharmaceuticals & Nutraceuticals — Excipients

Formulation & Processing Aids

Applications & Uses

Markets

Healthcare & Pharma

Applications

Healthcare & Pharma — Medical

Laboratory & Diagnostics

Preparation

Solution: IU per 1 mL solution is around 8,000～10,000 units.

Properties

Typical Properties

	Value	Units	Test Method / Conditions
Specific Activity	60.0	IU/mg protein	-

Contaminants

	Value	Units	Test Method / Conditions
Glucose-6-phosphate dehydrogenase	max. 0.0002	%	-
Phosphogluconate dehydrogenase	max. 0.001	％	-
Glutamate dehydrogenase (NAD+)	max. 0.0003	%	-
Glutathione reductase	max. 0.0002	%	-
NADPH oxidase	max. 0.003	%	-

Technical Details & Test Data

Reference Data

Reaction Equation

L-Glutamate + H20 + NADP+ = 2-Oxoglutarate + NH3 + NADPH

Assay Procedure

Spectrophotometric Method

1. Wavelength: 340 nm
2. Light path length: 1 cm
3. Temperature: 25℃
4. Pipette the following reagents into a cuvette

• 2.50 mL Triethanolamine･HCl-NaOH buffer (0.1 mol/L, pH 7.6)
• 0.15 mL α-Ketoglutarate(0.1 mol/L)
• 0.05 mL NADPH (12 mmol/L)
• 0.30 mL Ammonium acetate (2 mol/L)
• 0.02 mL rGlDH (NADPH) (about 3 IU/mL)

Packaging & Availability

Packaging Type

Bulk

Packaging

OYC No.	Package
46764004	1,000 units
46765004	3,000 units
46766004	15,000 units
46764904	Bulk

 

Storage & Handling

Storage Condition

1~10ºC