OYC Americas Hexokinase Grade I (HK- Y)

Identification & Functionality

Pharma & Nutraceuticals Functions

Enzyme

Technologies

Pharmaceuticals & Nutraceuticals

Product Families

Pharmaceuticals & Nutraceuticals — Excipients

Formulation & Processing Aids

Applications & Uses

Markets

Healthcare & Pharma

Applications

Healthcare & Pharma — Medical

Laboratory & Diagnostics

Assay Procedure

Spectrophotometric Method

1. Wavelength；340 nm
2. Light path length；1 cm
3. Temperature；25℃
4. Pipette the following reagents into a cuvette

• 2.40 mL Triethanolamine-HCl-NaOH buffer （0.1 mol/L, pH 7.5） containing Glucose（50 mg/mL）
• 0.30 mL MgCl2（0.1 mol/L）
• 0.15 mL ATP（10 mmol/L）
• 0.15 mL NADP+ （10 mmol/L）
• 0.01 mL G-6-PDH（500 IU/mL）
• 0.02 mL HK（about 3 IU/mL）

Preparation

Product Code：HK-93 Lyophilized powder (contains no ammonium sulfate)

IU per 1 mg powder is approximately 100 units.

Properties

Physical Form

Powder,

Solid

Typical Properties

	Value	Units	Test Method / Conditions
Specific Activity	min. 140	IU/mg protein	-

Contaminants

	Value	Units	Test Method / Conditions
Phosphoglucose isomerase	max. 0.1	％	-
Glutathione reductase	max. 0.005	%	-
Myokinase	max. 0.001	%	-
Phosphogluconate dehydrogenase	max. 0.005	％	-
Phosphoglucomutase	max. 0.01	-	-
Glucose-6-phosphate dehydrogenase	max. 0.01％	%	-
Creatine kinase	max. 0.005	%	-
ATPase	max. 0.003	%	-

Technical Details & Test Data

Reaction Equation

ATP ＋ D-Hexose ＝ ADP ＋ D-Hexose 6-phosphate

Packaging & Availability

Packaging Type

Bulk

Packaging

Lyophilized

OYC No.	Package
46560023	500 units
46561023	2,500 units
46562023	10,000 units
46563993	Bulk

 

Storage & Handling

Storage Condition

2 - 10°C