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LYSOCH® E4 Lysozyme is an antimicrobial enzyme extracted from egg-whites. It acts to kill gram- positive bacterial cells in processed foods by lysing their cell wall. . It is a safe and natural Lysozyme, which effectively prevents late blowing, by killing Clostridium tyrobutyricum in cheese production.

Ingredient Name: Lysozyme

Functions: Anti-Microbial Agent

Physical Form: Powder, Solid

Labeling Claims: Halal, Kosher, Natural, Non-GMO

Features: Anti-Bacterial, Antimicrobial, Enhanced Shelf Life, Growth control of Gram-positive Bacteria

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Identification & Functionality

Ingredient Name
Food Ingredients Functions
Ingredients
Lysozyme
Technologies

Features & Benefits

Key Benefits
  • Growth control of Gram-positive bacteria
  • Inactive against yeasts, molds and Gram- negative bacteria
  • Shelf life extension
  • Natural, Consumer-friendly label

Applications & Uses

Food & Nutrition Applications
Use Level
0.02 - 0.25 g/kg
Application Areas
  • Cheese, Wine, Beer
  • Microbiological stabilization after winemaking is one of the process that worry winemakers the most. Once the wine is ready to age or to be bottled, the enologist must maintain the quality of the final product.  Lysoch™ E4 can also control the onset of malolactic fermentation (MLF) and can contribute to the microbiological stability of wines, once alcoholic fermentation and/or MLF are completed.
  • LAB and yeast contamination, if left uncontrolled, can cause incidents in beer like turbidity, off flavor and ropiness. Lysoch™ E4 is effective at controlling LAB and contaminant yeasts (Brettanomyces) to extend the shelf life of high fermentation, non-pasteurized, and bottle-conditioned beers.
Direction For Use

The recommended dosage for Lysoch® E4 is in the range of 0.02 - 0.25 g/kg. In general, dissolve Lysoch® E4 into distilled cold boiled water, then add directly into foods. Exact dosage and way of application for Lysoch® E4 depends on the nature of the product for which it is intended.

Food Applications
Applications Benefits Dosage
Dairy Cheese Lysis of Clostridium tyrobutyricum 20 - 40 mg/kg
Beverage Wine Lysis of lactic acid bacteria and control of malolactic fermentation (MLF) 100 - 200 mg/L

Properties

Physical Form
Solubility
Appearance
Cream white powder
Typical Properties
ValueUnitsTest Method / Conditions
Activity in FIP (Units/mg Protein)min. 40,000IU/mg
Physico-Chemical Properties
ValueUnitsTest Method / Conditions
Solubility (at 640nm of a 2% Aqueous Solution)min. 95%
pH (2% Aqueous Solution at 20-25°C)3.0 - 3.6
Microbiological Values
ValueUnitsTest Method / Conditions
Total Plate Countmax. 1,000CFU/g
E. coliAbsent/25g
EnterobacteriaceaeAbsent
Salmonella spp.Absent/25g
Staphylococcus aureusAbsent
Composition
ValueUnitsTest Method / Conditions
Lysozyme (On the anhydrous basis)min. 99W%
Heavy Metals
ValueUnitsTest Method / Conditions
Arsenicmax. 1mg/kg
Mercurymax. 1mg/kg
Leadmax. 2mg/kg
Note

The exact appearance, activity and composition percentage variations may occur from batch to batch.

Regulatory & Compliance

Regulations
  • GMO Status -  According to regulations EC No. 1829/2003 and 1830/2003. Lysoch® E4 has not been derived from and does not contain compounds from Genetically Modified Origin.
  • In Cheese, Lysozyme is considered as generally recognized as safe (GRAS) by the U.S. FDA and approved as a preservative (E1105) under the European Additives Directive to prevent late blowing in ripened cheese. Lysozyme is also approved for use in cheese in Australia, Canada, China, Japan and South Korea.
  • In wine, OIV has approved Lysozyme as active ingredient up to the level of 500 mg/kg. The European Union has approved the use of Lysozyme as of October 26, 2001.
  • Packaging plastic materials comply with Regulation (EU) No 1935/2004, 2023/2006 and 10/2011.

Packaging & Availability

Packaging Type
Packaging

Lysoch® E4 is available in 500 g/bottle or 10 kg/barrel with integral, tamper-proof seals.

Storage & Handling

Shelf Life
24 Months
Storage

The shelf life is 24 months when stored unopened at room temperature in dry conditions, away from direct sunlight and odorous products. When opened, store at room temperature in the original container.