Knowde Enhanced TDS
Identification & Functionality
- Ingredient Name
- Ingredient Origin
- Food Ingredients Functions
- Pharma & Nutraceuticals Functions
- Ingredients
- Egg
Features & Benefits
- Food Ingredients Features
- Advantages & Benefits
As one of the largest manufacturers and suppliers worldwide of egg white proteins, Bioseutica®’s advanced extraction and purification processes yield products that are consistently chosen over other competitive products. All extraction and refining are done under cGMP guidelines for active pharmaceutical ingredients (API).
An important advantage of using Bioseutica®’s OVOTRANSFERRIN is the elimination of the risk of disease associated with bovine and human-derived transferrins.
Applications & Uses
- Markets
- Applications
- Food & Nutrition Applications
- Applications
Antibacterial
Among the several protective functions of OVOTRANSFERRIN, the most important one is likely to be the antibacterial activity, which is directly related to the OVOTRANSFERRIN's ability to bind iron (Fe3+), making it unavailable for bacterial growth. This bacteriostatic activity can be enhanced by adding carbonate ion which is one of the iron ligands in the OVOTRANSFERRIN metal-binding site increasing the pH from 6 to 8 and immobilizing OVOTRANSFERRIN by covalent linkage to Sepharose 4B [30]. An increase of the bacteriostatic activity towards E. coli O157:H7 as iron chelator was demonstrated using a combination of ovotransferrin, NaHCO3, and EDTA.Functional Food
OVOTRANSFERRIN shows a protective function similar to those already ascertained for the homologous mammalian lactoferrin. These additional functions, in many cases not directly related to iron binding, are also displayed by the peptides derived from partial hydrolysis of ovotransferrin. Several defensive properties of OVOTRANSFERRIN are also possessed by its proteolytic fragments, these properties may be of importance for human wellness and its derivatives as dietary additives in normal and pathological human conditions.(1)Research
Transferrins have been identified as a required media ingredient for the maturation of cells. The role of transferrins in culture systems is to provide iron to cells and to detoxify the media by binding contaminating metal ions, such as zinc, iron, and aluminum in their di and trivalent forms.Cardiovascular Disease
OVOTRANSFERRIN (OTRF, also referred to as conalbumin) and OTRF-derived peptides, including IRW, IQW, and KVREGT, are, by virtue of their anti-inflammatory and antioxidant characteristics, viable treatment agents for endothelial dysfunction and the prevention of CVD (2).Anticancer Drugs
In spite of the numerous discoveries of new anticancer drugs that work on different intracellular targets, many of these drugs have a considerable drawback as they are nonselective thus causing adverse side effects on normal cells. A new study is aimed to explore the potential of ovotransferrin (OTf) as a carrier molecule to allow specific targeting of anticancer drugs to cancer cells via the transferrin receptor (TfR). Receptor binding assay provided evidence that OTf bind to the transferrin-receptor (TfR) of human colon cancer cells. Two anticancer drugs, carboplatin (cbp) and paclitaxel (PTX) were non-covalently conjugated with OTf, at different mole ratios, through freeze-condensation. The two OTf conjugates showed superior anti-proliferative activities against human colon carcinoma (HCT-116) and human breast carcinoma (MCF-7) cells compared to the activities of free drugs, with OTf-cbp being the most potent conjugate. The conjugates with low drug loading inhibited cell growth more efficient than the high drug-loaded conjugates. Fluorescence staining with acridine orange and propidium iodide showed that HCT-116 cells treated with OTfcbp or OTf-PTX exhibited red fluorescence, indicating that PI entered to the nuclei as the cell membrane lost its integrity. The red fluorescence was accompanied by chromatin condensation and fragmentation indicates apoptosis. The results demonstrate, for the first time, that OTf could be utilized to specifically target drugs via TfR-mediated endocytosis to cancer cells and will help to pave the way for clinical studies as a potential targeting molecule (3).Skincare
OVOTRANSFERRIN belongs to a group of proteins called metalloproteinases that have been found to induce the production of heat shock proteins (HSPs). Clinical trials with OVOTRANSFERRIN have found that it is able to induce HSPs in the skin, which provides protection against cold stress and other environmental factors. HSPs play a fundamental role in life-essential processes and are associated with many areas of biology and medicine. They are known to help cells disassemble and dispose of damaged proteins and to help in the making and transport of new proteins. The role of HSPs in the aging process is not yet clear. However, the ability of OVOTRANSFERRIN to induce HSP production suggests it can be effectively utilized in personal care skin creams and moisturizers.Nutrition
OVOTRANSFERRIN, like serum transferrin, is also similar in structure and function to lactoferrin from milk. However, OVOTRANSFERRIN can be extracted in larger quantities and is suitable for use in therapeutic programs. The addition of OVOTRANSFERRIN to cow’s milk (which is generally low in transferrins) enhances its antibacterial property and makes it comparable to human milk.
Technical Details & Test Data
- Comparison of Amino Acids Profiles
These same investigators later determined that a fraction of human blood serum exerts the same iron-binding action as OVOTRANSFERRIN. This blood serum protein was initially named siderophilin, but today is known as human transferrin. OVOTRANSFERRIN and blood transferrin are now known to have similar amino acid compositions (see table), as well as similar carbohydrate content.
Amino Acids Ovotransferrin Human Transferrin Bovine Serum Transferrin Lysine 53 51 52 Histidine 13 19 15 Arginine 33 24 24 Aspartic Acid 77 81 74 Threonine 38 30 44 Serine 46 40 49 Glutamic Acid 62 58 63 Proline 33 30 35 Glycine 58 54 45 Alanine 38 59 49 Half Cystine 12 35 34 Valine 54 43 44 Methionine 10 4 7 Isoleucine 29 14 17 Leucine 51 47 45 Tyrosine 19 24 20 Phenylalanine 27 28 25 Tryptophan 11 9 10