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Hispanagar LM GQT AGAROSE

This agarose, with a GQT (Genetic Quality Tested) certificate, is especially recommended for preparatory nucleic acids electrophoresis ≥1 000 pb and in-gel applications. The presence of DNase and RNase is not detected and does not have DNA Binding properties.

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Electrophoresis Gel, Gelling Agent

Physical Form: Gel, Powder, Solid

Safety Data Sheet

Enhanced TDS

Knowde-enriched technical product data sheet

Identification & Functionality

Chemical Name
Agarose
Pharma & Nutraceuticals Functions
Electrophoresis Gel,
Gelling Agent
CAS No.
9012-36-6
EC No.
232-731-8
Technologies
Pharmaceuticals & Nutraceuticals
Product Families
Pharmaceuticals & Nutraceuticals — Processing, Purification & Testing Solutions
Purification & Processing Aids

Applications & Uses

Markets
Healthcare & Pharma
Applications
Healthcare & Pharma — Pharmaceutical Manufacturing
Laboratory & Research
Applications
  • Electrophoresis of DNA fragments ≥1000 bp.
  • In-Gel enzymatic processing (digestion, ligation, PCR).
  • Preparative electrophoresis.
  • Analysis and recovery of large DNA fragments for further applications.

Properties

Physical Form
Gel,
Powder,
Solid
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%-
End-Expiratory Occlusionmax. 0.12--
Gel Strength (at 1%)min. 250g/cm2-
Gelling Temperature (at 1.5%)24-28°C-
Melting Temperature (at 1.5%)max. 65.5°C-
Moisture Contentmax. 10%-
Sulfate Contentmax. 0.1%-

Technical Details & Test Data

Functional Tests
  • DNA resolution: bands appear sharp and finely resolved.
  • DNAse/RNAse activity: none detected.
  • DNA binding: none detected.
  • In-Gel enzymatic processing: passes test.
  • Enzymatic degradation by agarase: passes test.
  • Gel background: very low after EtBr staining.
Test Data

Hispanagar LM GQT AGAROSE Test Data

LM GQT Agarose at different concentrations. A-0.75%, B-1% and C-1.25%.
Marker: 1Kb ladder, 0.5 μg/lane.
Running conditions: 1X TAE buffer, 4,5V/cm, 2 hours 30 min.

Ranges Of Separation For LM AND LM GQT AGAROSE

Hispanagar LM GQT AGAROSE - Ranges of Separation For Lm And Lm Gqt Agarose

Range of separation depends upon the choice of buffer. The ranges were determined in presence of TAE buffer. Migration in the TBE buffer is slower; therefore lower concentrations can be used to obtain similar separation ranges.