company tower banner
Hispanagar Company Logo

Hispanagar D-1 LE GQT AGAROSE

Highly purified agarose with low electroendosmosis values ranging from 0.05 to 0.13. This product has a GQT (Genetic Quality Tested) quality control certificate and is especially recommended for DNA preparation gels. The presence of DNase and RNase is not detected.

Ingredient Name: Agarose

Chemical Name: Agarose

CAS Number: 9012-36-6

Functions: Bulking Agent, Carrier, Electrophoresis Gel, Emulsifier, Gelling Agent, Glazing Agent, Humectant, Stabilizer, Thickener, Viscosity Modifier

Labeling Claims: Non-Toxic

Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan

Safety Data Sheet
  • TypeDocument Name
View All Documents

Knowde Enhanced TDS

Identification & Functionality

Chemical Name
Ingredient Name
Pharma & Nutraceuticals Functions
CAS No.
9012-36-6
EC No.
232-731-8

Features & Benefits

Labeling Claims
Features
  • Extraordinary mechanical resistance for more reliable and easier handling.
  • Possibility of varying pore size in accordance with particle size by modifying the gel concentration.
  • Easy preparation of the gel by simple dissolution in aqueous buffers either by standard boiling or microwaving.
  • Greater thermal stability due to high hysteresis.
  • Excellent transparency of the gels.
  • Low absorption of staining agents.
  • Absence of toxicity.

Applications & Uses

Applications
  • Analytical and preparative gel electrophoresis for nucleic acids ≥ 1000 bp.
  • Blotting assays.
  • Recovery of DNA fragments for further applications (enzymatic processing or cloning).

Properties

Physical Form
Typical Properties
ValueUnitsTest Method / Conditions
Ash Contentmax. 0.4%
Clarity (at 1.5%)max. 3NTU
End-Expiratory Occlusion0.05-0.13
Gel Strength (at 1.5%)min. 2500g/cm2
Gel Strength (at 1%)min. 1200g/cm2
Gelling Temperature (at 1.5%)36 ± 1.5°C
Melting Temperature (at 1.5%)88 ± 1.5°C
Moisture Contentmax. 10%
Sulfate Contentmax. 0.1%

Regulatory & Compliance

Certifications & Compliance

Technical Details & Test Data

Test Data

Hispanagar D-1 LE GQT AGAROSE - Test Data

D1-LE Agarose gels in IX TAE buffer A-0.75%, B-1%, C-1.25%
Marker: 1Kb ladder.
Electrophoresis conditions: submarine gel, 2 hours 30 min, 4.5 V/cm in 1X TAE buffer.

Functional Tests
  • DNA resolution: bands appear sharp and finely resolved.
  • DNAse/RNAse activity: none detected.
  • DNA binding: none detected.
  • Gel background: very low after EtBr staining.
  • Inhibition to restriction enzymes and ligase: none detected.
Ranges Of Separation For D-1 LE And D-1 LE GQT AGAROSE

Hispanagar D-1 LE GQT AGAROSE - Ranges of Separation For D-1 Le And D-1 Le Gqt Agarose